| PRKG1

cGMP-dependent protein kinase 1, alpha isozyme is an enzyme that is encoded by the PRKG1 gene.Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. Diseases associated with PRKG1 include Aortic Aneurysm, Familial Thoracic 8 and Familial Thoracic Aortic Aneurysm And Aortic Dissection.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.34	1.66	1.07	0.68	0.43	0.32	0.23	0.1
Corrected OD	2.24	1.56	0.97	0.58	0.33	0.22	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PRKG1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	96-107	104

 

| Linearity

Samples were spiked with high concentrations of Human PRKG1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95
	Average (%)	91
1 : 4	Range (%)	89-103
	Average (%)	94